The aim of this study is to investigate the effects of different lateral spacing and frequencies of nitrogen (N) fertigation on lint yield and content of some macro and micro elements for drip-irrigated cotton. The split-plot experimental design was applied in this research. The main plots; L1:1 lateral for each row (lateral space are 0.70 m.), L2:1 lateral for 2 rows (lateral space is 1.40 m); Sub-plots were designed as different application N frequencies; F1: N application for each irrigation (5 days) F2: N application for each 2 irrigations (10 days) F3: N application one-fourth of total N between the first irrigation and the first flowering, two-fourth between the first flowering and the first boll formation, and one-fourth between the first boll formation and the last irrigation. The maximum cotton lint yield (1855 kg ha-1) was obtained from the treatment of 1 lateral for 2 rows and each 2 irrigations (10 day). The total N content in cotton leaves ranged from 1.66 to 3.05 % in 2011 and from 1.92 to 3.01% in 2012 depending on experimental treatments and growing stages of the plants. The uptake of N increased with an increasing frequency of N application. The maximum NUE (11.1-17.4 kg lint yied ha-1 kg N-1) was obtained for N fertigation every two irrigation cycles (10 days). The contents of other some macro and micro element in the cotton leaves were: P : 0.19-0.27 %, K: 1.56-1.72 %, Ca: 2.6-5.6 %, Mg: 0.13-0.37 %, Cu: 6.03-8.19 ppm, Zn: 3.55-15.81 ppm, Mn: 43.90-91.92 ppm and Fe: 161-381 ppm. There were no significantly defficiency in terms of micro elemenst except Zn for cotton crop.