The Research was conducted at International Agricultural Research and Training Centre in Menemen, located western Turkey, in the late summer period in 2014. In this research, lettuce was irrigated by 3 different irrigation methods, drip irrigation, subsurface drip irrigation and furrow irrigation, with treated domestic waste water. Anion-cation, pollution analyses and fecal coliform tests were run. Also soil samples, from plots from the depth of 0-20 cm, were taken and analyzed in order to monitor the status of the pathogens in soil. Water`s pH values were 7.59-7.79, EC values were 2.7-3.9 dS m^-1, fecal coliform values variety 1.456x103-2.568 x103 cfu 100 ml^-1. According to the analyses of soil samples before irrigation by treated waste water salmonella was also uncounted beforehand. It is thought that, this case can be a result of the feces of winged living around the trial area. According to the pathogenic analyses run on harvested lettuce E. coli values were found respectively 2x101 cfu 100g^-1 for sub-surface drip irrigation, 3 x101 cfu 100g^-1 E. for drip irrigation and 4.4 x102 cfu 100g^-1 for furrow irrigation. As a conclusion, it was found that pathogenic contamination in subsurface drip irrigation is less than the other methods.