The aim of this study is to find out whether there is chemical and microbiological degradation in the samples taken from fresh water sources near and far from high voltage lines. The samples were taken from Erfelek Meydan Village location Karasu River, Ayancık Tevfikiye Village location Zindan Brook, Ayancık Türkeli road junction Ayancık Brook, Durağan Yalnız Kavak location Gökırmak Water, Durağan- Boyabat Yalnız Kavak location Gökırmak water and Boyabat Sinecan Village location Kırkgeçit Brook in Sinop province of Turkey. Na⁺, K⁺, Cl^-, pH and conductivity analysis were conducted chemically, while microbiological tests were made against Escherichia coli and coliform bacteria. All the results obtained were assessed according to 06.07.2019 dated and 30823 numbered `Legislation on the quality and treatment of water from which drinking water is obtained` in T. C. Official Gazette. With this study, it can be said that high voltage lines in the province of Sinop do not have a significant effect on fresh water sources. Na⁺, K⁺, Cl^- gave results in expected levels and conductivity gave results directly proportional to pH. In microbiological test results, Escherichia coli and coliform bacterial growth was found, while no microbiological degradation was found.