This study was conducted to determine the effects of the zinc (Zn) EDTA (Ethylenediaminetetraacetic acid) and Zn sulphate (ZnSO4.7H2O) sources on the yield and zinc content of seed at flowering and harvest stage of safflower. Zinc sources were applied in the forms of control, Zn-EDTA in soil, Zn-EDTA foliar spray, Zn-EDTA in soil + foliar spray, ZnSO4 in soil, ZnSO4 foliar spray and ZnSO4 in soil + foliar spray. The safflower cv. Remzibey-05 was used as material. The head number per plant, the seed yield and the zinc concentration and content of stem+leaf and seed at flowering and harvest stage were investigated. According to the results, different sources and applications of zinc increased seed yield when it compared with control. The application of Zn-EDTA in soil+foliar spray increased the seed yield 21% whereas ZnSO4 in soil + foliar spray increased seed yield 16 % when it compared with the control. As a result, the highest seed yield and zinc content in seed of safflower were determined by the application of Zn-EDTA in soil+foliar spray.