This paper presents the results of testing the quality of water for irrigation during the growing seasons 2012/2013, in the basin of the Kolubara River, from Beloševac to Obrenovac, in three monitoring cycles on 16 selected sites belonging to agricultural area under irrigation. The determination of quantity of trace elements and heavy metals Cr, Ni, Pb, Cu, Zn, Cd, B, As, Fe, Hg was performed during mentioned period. The content of trace elements and heavy metals in the samples of water is generally below the maximum allowable concentration (MAC). In the samples No. 2, 3, 4, in the second series of sampling was recorded higher content of As above the MAC, which can be explained by drought, low water levels and potential anthropogenic pollution. In the other two cycles of monitoring are not registered concentrations of tested elements above MAC. Based on the presented and analyzed results of testing of hazardous and harmful substances in the water for irrigation of the Kolubara River, it can be concluded that it can be used for irrigation of crops and soil with restrictions and frequent quality checks during the summer months and control potential sources of pollution from industry.