In this study, it was aimed to determine and mapping of soil microbial activities in widespread soil series of Amik Plain. For this purpose, soil samples were taken from 11 soil series (Aşağıoba, Karasu, Topboğazı, Acarköy, Comba, Reyhanlı, Sazyurdu, Asi, Keçebek, Arpahan, Akkerpiç) in the autumn period. Each will represent a series of five sampling points and 0-30 cm depth of soil samples taken from the three paralleled without waiting is subjected to analysis. Coordinates of sampling points were recorded with the help of handheld GPS. Soil respiration (CO₂), dehydrogenase enzyme activity (DHA) and microbial biomass carbon (MBC) analyzes were done for the purpose of determined of microbial activity. Thematic maps were produced as a result of the analysis of the values placed basis of soil mapping units of related series. According to research results, the microbial point of the highest CO₂, DHA and MBC values were determined as 29.0 mg CO₂ 100g.ds/24 h. in Acarköy Series, 1119 μg TPF 10 g.ds/24h. in Reyhanlı Series and 256 mg C/kg.ds. in Asi Series` soils respectively. Belong to the same parameters of the lowest values were determined as 11.4 mg CO<sub>2</sub> 100 g.ds/24sa. in Aşağıoba Series, 392 μg TPF 10 g.ds/24h. in Acarköy Series and 121 mg C/kg.ds. in Reyhanlı Series` soils respectively.